The Primary Structure of Porcine Pancreatic Ribonuclease
نویسندگان
چکیده
منابع مشابه
The primary structure of porcine pancreatic ribonuclease. I. The distribution and sites of carbohydrate attachment.
Reduced, S-aminoethylated porcine ribonuclease was subjected to tryptic hydrolysis. A sequence of fractionation steps that included gel filtration and chromatography over SE-Sephadex C-50, Dowex 50-X2, and Dowex l-X2 afforded a series of glycopeptide fractions which were recognized to derive from three separate regions of the molecule. Heterogeneity of the attached polysaccharide chains preclud...
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Porcine pancreatic RNase was isolated from the pancreatic secretion of animals with permanent fistulas by a combination of DEAE-cellulose chromatography, adsorption-elution from sulfoethyl-Sephadex, and gel filtration on Sephadex G-100. On the basis of these experiments, a practicable, large scale procedure was developed for the preparation of the enzyme in quantities of 100 to 200 mg. Porcine ...
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The primary structure of pancreatic ribonuclease from langur (Presbytis entellus) has been determined. This sequence differs from that of human pancreatic ribonuclease at 14 (11%) of the amino acid positions. Eight of these 14 differences involve changes of charge, with the langur enzyme having five fewer positive charges than the human enzyme. The difference in charge between human and langur ...
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15 صفحه اولOn the Structure of Bovine Pancreatic Ribonuclease B
Ribonuclease B is a constituent of the zymogen granules of bovine pancreatic acinar cells and is secreted in the pancreatic juice of cattle (2). Its isolation from bovine pancreatic juice has been described (3). The enzyme is a glycoprotein that possesses an amino acid composition indistinguishable from that of ribonuclease A and contains carbohydrate to the extent of 6 residues of mannose and ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1970
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)63378-2